Monoclonal anti-mouse laminin antibodies: AL-1 reacts with laminin α1 chain, AL-2 with laminin β1 chain, and AL-4 with the coiled-coil domain of laminin β1 chain
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چکیده
include the final publisher proof-corrections or journal pagination. Monoclonal anti-mouse laminin antibodies: AL-1 reacts with laminin alpha1 chain, AL-2 with laminin beta1 chain, and AL-4 with the coiled-coil domain of laminin beta1 chain. Access to the published version may require journal subscription. Abstract We analyzed the reactivity of three different commercially available rat monoclonal antibodies raised against mouse laminin-α1β1γ1 (laminin-111), AL-1, AL-2, and AL-4. Using ELISA assays, Western blot analysis and immunostainings we present refined epitope maps for these three laminin monoclonals. AL-1 reacted, as predicted with laminin α1 chain. AL-4 has also been marketed as an α1 chain specific probe, but we show here that AL-4 detects mouse laminin β1 chain, in the distal part of the coiled-coil region. AL-2 was predicted to react with all three chains near the cross region, but seems to primarily react with laminin β1 chain.
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